Fig. 7
Effects on the cell wall of S. cerevisiae YCPUC209C variant. (A) Sensitivity to CFW or CR YCPUC209 strain and YCPUC209C variant. Cells were incubated in malt medium in the presence or absence of 25–50 µg/mL CFW or CR for 72 h at 20 °C. (B) Zymolyase sensitivity. YCPUC209 strain and YCPUC209C variant were incubated with 200 µg/mL zymolyase 20T at 25 ºC, and the decrease in OD600 nm was monitored over time (survival %). (C) The YCPUC209C strain presented a reduction in budding cell number.
