Table 1 Regression and validation data for the determination of lacidipine by the proposed first derivative synchronous spectrofluorimetric procedure. a the peak amplitude of the first derivative of synchronous fluorescence spectra.b concentration of Lacidipinein ng/ml.c average of nine determinations (three concentrations repeated three times).d %RSD of nine determinations (three concentrations repeated three times).e average of three determinations.

From: Green first derivative synchronous spectrofluorimetric determination of lacidipine and its acid degradation product in plasma and mixtures

Parameters

Proposed method

Wavelength (nm)

409

Concentration range (ng/mL)

50 ─ 300

LOD (ng/mL)

14.51

LOQ (ng/mL)

43.97

Regression Equation

- Slope (b)

- Intercept (a)

y = 0.1138x + 0.4045

0.1138

0.4045

Coefficient of determination (r2)

0.9997

Accuracy (% R)c

100.21

Precisiond (% RSD)

-Repeatability

-Intermediate precision

1.34

1.12

Robustness (% Re ±% RSD)

- Δλ(± 1 nm)

- pH (± 0.1)

- Britton Robinson buffer volume (± 0.1 mL)

98.53 ± 0.92

99.47 ± 1.63

101.25 ± 1.21

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