Table 1 Phycoerythrin pigment purification and recovery steps.
Step | Volume (mL) | PE (µg mL−1) | Purity (A620/A280) | Recovery (%) |
---|---|---|---|---|
Crude pigment | 100 | 78.43 | 0.95 | 100 |
Ammonium sulfate precipitation of pigment | 15 | 151.24 | 2.24 | 74 |
Dialysis of pigment | 10 | 338.71 | 3.39 | 35 |
Sephadex G-100 Column chromatography of pigment | 5 | 554.12 | 5.63 | 11 |