Table 2 Primers used for identification of plasmid-encoded virulence genes in the studied STEC isolates.

From: Shiga toxin-producing Escherichia coli plasmid diversity reveals virulence potential and control opportunities in animal hosts

Primer name

Sequence (5’-3’ direction)

Amplicon size (bp)

Annealing tm/timea

Reference

Virulence genes

astA

CCATCAACACAGTATATCCGA

111

55 °C/60 sec

53

 

GGTCGCGAGTGACGGCTTTGT

   

cma

CCAGCAACCCTCTCACATTGCAG

556

67 °C/60 sec

54

 

CCAGAAAACATCGCCCCGAGCC

   

cba

TCGCTCCATCCATGCCTCCG

138

66 °C/60 sec

 
 

CCATCCCGACCAGTCTCCCTC

   

T6-rhsA

CGCTATCTTTACGACCCGCT

  

26

T6-rhsA1

GGCAAGGGGAATGGTCTAGG

800

57 °C/60 sec

 

T6-rhsA2

GATGTGGGGGTACCATGCC

875

57 °C/60 sec

 

T6-rhsC

TAGGCGGTTTGTTGGGTCTC

832

57 °C/60 sec

 

stcE

GGCTCCGGAGGTGGGGGAAT

399

58 °C/60 sec

55

 

GAAGCCGGTGGAGGAACGGC

   

katP

GCGGAAGAGAAGATGACTGG

277

55 °C/60 sec

56

 

GCACCATGTGCTTTACCAAA

   

toxB

ATACCTACCTGCTCTGGATTGA

602

51 °C/60 sec

57

 

TTCTTACCTGATCTGATGCAGC

   

espP

TTGCGAAAAATGGCGGAACTC

956

55 °C/60 sec

58

 

CGGAGTCGTCAGTCAGTAGA

   

subAB

GCAGATAAATACCCTTCACTTG

232

56 °C/60 sec

59

 

ATCACCAGTCCACTCAGCC

   

IhaG

AACTGGCAGATCACCGAAGA

346

55 °C/60 sec

29

 

GCGACATCCAGTAATTTCGCT

   

LAA iha

TTTCAGCCAGCAGCATGGCA

172

55 °C/60 sec

 
 

ACATCCACACCCTCCACAGC

   

saa

CGTGATGAACAGGCTATTGC

119

56 °C/60 sec

51

 

ATGGACATGCCTGTGGCAAC

   

T2-etpD

CGTCAGGAGGATGTTCAG

1061

53 °C/60 sec

38

 

CGACTGCACCTGTTCCTGATTA

   

cif

AACAGATGGCAACAGACTGG

383

54 °C/60 sec

59

 

AGTCAATGCTTTATGCGTCAT

   

epeA

CACCCTGTAGAATCTTA

1873

56 °C/60 sec

52

 

CTGAATAAATCCAGCCC

   
  1. a Annealing temperature and duration.