Table 2 Primers used for identification of plasmid-encoded virulence genes in the studied STEC isolates.
Primer name | Sequence (5’-3’ direction) | Amplicon size (bp) | Annealing tm/timea | Reference |
|---|---|---|---|---|
Virulence genes | ||||
astA | CCATCAACACAGTATATCCGA | 111 | 55 °C/60 sec | 53 |
| Â | GGTCGCGAGTGACGGCTTTGT | Â | Â | Â |
cma | CCAGCAACCCTCTCACATTGCAG | 556 | 67 °C/60 sec | 54 |
| Â | CCAGAAAACATCGCCCCGAGCC | Â | Â | Â |
cba | TCGCTCCATCCATGCCTCCG | 138 | 66 °C/60 sec |  |
| Â | CCATCCCGACCAGTCTCCCTC | Â | Â | Â |
T6-rhsA | CGCTATCTTTACGACCCGCT | Â | Â | 26 |
T6-rhsA1 | GGCAAGGGGAATGGTCTAGG | 800 | 57 °C/60 sec |  |
T6-rhsA2 | GATGTGGGGGTACCATGCC | 875 | 57 °C/60 sec |  |
T6-rhsC | TAGGCGGTTTGTTGGGTCTC | 832 | 57 °C/60 sec |  |
stcE | GGCTCCGGAGGTGGGGGAAT | 399 | 58 °C/60 sec | 55 |
| Â | GAAGCCGGTGGAGGAACGGC | Â | Â | Â |
katP | GCGGAAGAGAAGATGACTGG | 277 | 55 °C/60 sec | 56 |
| Â | GCACCATGTGCTTTACCAAA | Â | Â | Â |
toxB | ATACCTACCTGCTCTGGATTGA | 602 | 51 °C/60 sec | 57 |
| Â | TTCTTACCTGATCTGATGCAGC | Â | Â | Â |
espP | TTGCGAAAAATGGCGGAACTC | 956 | 55 °C/60 sec | 58 |
| Â | CGGAGTCGTCAGTCAGTAGA | Â | Â | Â |
subAB | GCAGATAAATACCCTTCACTTG | 232 | 56 °C/60 sec | 59 |
| Â | ATCACCAGTCCACTCAGCC | Â | Â | Â |
IhaG | AACTGGCAGATCACCGAAGA | 346 | 55 °C/60 sec | 29 |
| Â | GCGACATCCAGTAATTTCGCT | Â | Â | Â |
LAA iha | TTTCAGCCAGCAGCATGGCA | 172 | 55 °C/60 sec |  |
| Â | ACATCCACACCCTCCACAGC | Â | Â | Â |
saa | CGTGATGAACAGGCTATTGC | 119 | 56 °C/60 sec | 51 |
| Â | ATGGACATGCCTGTGGCAAC | Â | Â | Â |
T2-etpD | CGTCAGGAGGATGTTCAG | 1061 | 53 °C/60 sec | 38 |
| Â | CGACTGCACCTGTTCCTGATTA | Â | Â | Â |
cif | AACAGATGGCAACAGACTGG | 383 | 54 °C/60 sec | 59 |
| Â | AGTCAATGCTTTATGCGTCAT | Â | Â | Â |
epeA | CACCCTGTAGAATCTTA | 1873 | 56 °C/60 sec | 52 |
| Â | CTGAATAAATCCAGCCC | Â | Â | Â |
