Table 2 Primers used for identification of plasmid-encoded virulence genes in the studied STEC isolates.
Primer name | Sequence (5’-3’ direction) | Amplicon size (bp) | Annealing tm/timea | Reference |
---|---|---|---|---|
Virulence genes | ||||
astA | CCATCAACACAGTATATCCGA | 111 | 55 °C/60 sec | 53 |
GGTCGCGAGTGACGGCTTTGT | ||||
cma | CCAGCAACCCTCTCACATTGCAG | 556 | 67 °C/60 sec | 54 |
CCAGAAAACATCGCCCCGAGCC | ||||
cba | TCGCTCCATCCATGCCTCCG | 138 | 66 °C/60 sec | |
CCATCCCGACCAGTCTCCCTC | ||||
T6-rhsA | CGCTATCTTTACGACCCGCT | 26 | ||
T6-rhsA1 | GGCAAGGGGAATGGTCTAGG | 800 | 57 °C/60 sec | |
T6-rhsA2 | GATGTGGGGGTACCATGCC | 875 | 57 °C/60 sec | |
T6-rhsC | TAGGCGGTTTGTTGGGTCTC | 832 | 57 °C/60 sec | |
stcE | GGCTCCGGAGGTGGGGGAAT | 399 | 58 °C/60 sec | 55 |
GAAGCCGGTGGAGGAACGGC | ||||
katP | GCGGAAGAGAAGATGACTGG | 277 | 55 °C/60 sec | 56 |
GCACCATGTGCTTTACCAAA | ||||
toxB | ATACCTACCTGCTCTGGATTGA | 602 | 51 °C/60 sec | 57 |
TTCTTACCTGATCTGATGCAGC | ||||
espP | TTGCGAAAAATGGCGGAACTC | 956 | 55 °C/60 sec | 58 |
CGGAGTCGTCAGTCAGTAGA | ||||
subAB | GCAGATAAATACCCTTCACTTG | 232 | 56 °C/60 sec | 59 |
ATCACCAGTCCACTCAGCC | ||||
IhaG | AACTGGCAGATCACCGAAGA | 346 | 55 °C/60 sec | 29 |
GCGACATCCAGTAATTTCGCT | ||||
LAA iha | TTTCAGCCAGCAGCATGGCA | 172 | 55 °C/60 sec | |
ACATCCACACCCTCCACAGC | ||||
saa | CGTGATGAACAGGCTATTGC | 119 | 56 °C/60 sec | 51 |
ATGGACATGCCTGTGGCAAC | ||||
T2-etpD | CGTCAGGAGGATGTTCAG | 1061 | 53 °C/60 sec | 38 |
CGACTGCACCTGTTCCTGATTA | ||||
cif | AACAGATGGCAACAGACTGG | 383 | 54 °C/60 sec | 59 |
AGTCAATGCTTTATGCGTCAT | ||||
epeA | CACCCTGTAGAATCTTA | 1873 | 56 °C/60 sec | 52 |
CTGAATAAATCCAGCCC |