Fig. 2
From: Sex dependent effects of GPR109A gene deletion in myeloid cells on bone development in mice
Best representative images from immunohistochemistry confirm deletion of GPR109A in tibia of both male and female CKO mice. (A, B) Immunohistochemistry was performed on tibia isolated from male and female f/f and CKO mice at 6 months of age. Loss of GPR109A protein expression (in red) was confirmed for CKO mice is present in tibia as well as the presence of LysM-Cre (in green). LysM and DAPI staining (in blue) are unaffected for CKO mice compared to f/f mice confirming myeloid cell deletion of GPR109A did not affect tibia at the cell level.
