Fig. 6

µCT analysis of female L5 vertebrae reveal later in life improvements in CKO trabecular bone compared to both f/f and Cre⁺ controls. (A) µCT parameters for L5 vertebrae from female mice (f/f, Cre⁺ and CKO) at different time points (35 days, 3 months, 6 months, 12 months). Impact of GPR109A myeloid cell deletion is primarily observed at later time points as observed by significant increases in BV, BV/TV and Tb N as well as a significant decrease in Tb Sp at both 6 and 12 months for CKO mice compared to both f/f and Cre⁺ controls. In addition, BS/TV was significantly increased for CKO mice at 12 months when compared to Cre⁺ but not f/f mice. Finally, at 6 months BMD for CKO mice was found to be significantly increased compared to f/f and Cre⁺ controls. (B) Line graph of female L5 vertebrae BMD over all time points showing peak BMD occurs at 6 months for CKO mice. For One-way ANOVA, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, **** P ≤ 0.0001.