Fig. 2

PjELNs significantly suppress inflammation-related pathways upregulated by LPS stimulation. (A) Samples used for RNA-seq analysis. (B) Volcano plots showing LPS versus None (left panel), and LPS-PjELNs versus LPS (right panel). Green dots represent significantly upregulated genes (FDR ≤ 0.05 and logFC > 0.6), while red dots represent significantly downregulated genes (FDR ≤ 0.05 and logFC < -0.6). (C) Venn diagram comparing upregulated genes in LPS versus None with downregulated genes in LPS-PjELNs versus LPS (upper panel). The bottom panel shows the results specifically for cytokines. (D) Heatmap of genes significantly differentially expressed in LPS-PjELNs versus LPS (Figure. 2B right panel). The upper panel displays gene expression levels standardized with z-scores across samples. The bottom panel shows the log fold change (logFC) values between the indicated conditions. (E) Boxplots of gene expression levels across conditions. (F,G) Dot plots showing Gene Set Enrichment Analysis (GSEA) results for pathways upregulated in LPS versus None (F) and downregulated in LPS-PjELNs versus LPS (G). Results are represented by size (gene count; legend in the upper right) and color (FDR; legend in the lower right). (H,I) Enrichment plots of the TNFA SIGNALING VIA NFKB (H) and IL6 JAK-STAT3 SIGNALING pathways (I) from Fig. 2F and G, respectively.