Fig. 8
From: Chlorogenic acid attenuates cardiac hypertrophy and fibrosis by downregulating galectin 3
Galectin 3 overexpression increased cardiomyocyte size. (A–B) Representative images of cells overexpressing pCMV3-N-GFPSpark-galectin 3 and quantification of cell size. Green fluorescence indicates GFP expression. Each merged image represents a combination of the three individual channels. Scale bar = 100 μm. (C) Nppa mRNA levels in galectin 3-overexpressing cells. (D–E) Representative immunoblot images and quantification of NPPA expression in galectin 3-overexpressing cells. **P < 0.01 and ***P < 0.001. GFP; green fluorescent protein. (F) Schematic diagram of the proposed mechanism showing how chlorogenic acid regulates cardiac hypertrophy and fibrosis.
