Fig. 7
CBS silencing promotes ferroptosis in STAD cells. (A) Ferroptosis-related proteins changed with the decrease of CBS. (B) Schematic representation of GSH involvement in the ferroptosis pathway. (C) The cytotoxicity of RSL-3 (2.5 µM) and DHA (100 µM) was tested after the treatment of 20 h treatment in both NC and siCBS cells. (D) Typical pictures were captured to measure intracellular ROS levels using an M7000 microscope after the application of RSL-3 (5 µM) in MKN-45 cells. (E) DCFH-DA-labeled ROS levels were quantified. (F) After 4 h of treatment with RSL-3 (5 µM), the cells were stained with BODIPY for 30 min and the fluorescence intensity was measured by flow cytometry. (G) BODIPY-labeled lipid peroxide levels were quantified.
