Correction to: Scientific Reports https://doi.org/10.1038/s41598-025-90532-z, published online 19 February 2025

The original version of this Article contained errors in Fig. 1, panel b and f, where the images of the crystal violet staining samples (NCI-H15685-FUR cells with 5 μM 5-FU in Fig. 1b, and MCF75-FUR cells with 20 μM 5-FU in Fig. 1f) were incorrectly used from the images of samples (Vector cells with 0 μM 5-FU, and AAVS1 cells with 0 μM 5-FU, respectively) in panel l.

Fig. 1
figure 1

Derivation of multiple 5-FU-resistant cell lines. (a) Cell growth evaluation of parental and 5-FU-resistant (5-FUR) lung cancer NCI-H1568 cells upon 5-FU treatment. Mean ± SD with n = 3. Unpaired t test, ***p < 0.001. (b) Crystal violet staining of parental and NCI-H15685-FUR cells. (c) MTT-based cell viability assay for parental and NCI-H15685-FUR cells. Mean ± SD with n = 3. (d) IC50 values of parental and NCI-H15685-FUR cells in response to 5-FU. (e–h) Evaluation results of cell growth (e), crystal violet staining (f), MTT assay (g) and IC50 values (h) for parental and breast cancer MCF75-FUR cells. (i-j) Ranked genes from genome-wide CRISPR knockout screen in NCI-H1568 (i) or MCF7 (j) cells against 5-FU. Red highlighted gene indicates a resistance phenotype upon gene knockout while blue indicates lethal upon 5-FU treatment. (k-l) Crystal violet staining showing a resistance phenotype to 5-FU when MMACHC or MED19 was knocked out using two independent sgRNAs (-1 and -2) in NCI-H1568 (k, 5 μM for 9 days) or MCF7 (l, 20 μM for 12 days) cells, respectively. (m–n) Cell growth evaluation of control (vector, sgAAVS1) and 5-FU-resistant (5-FUR) NCI-H1568 (m, 5 μM for 8 days) or MCF7 (n, 20 μM for 11 days) cells derived from MMACHC or MED19 knockout, respectively. Mean ± SD with n = 3. Unpaired t test (5-FU vs. DMSO), ***p < 0.001.

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The original Article has been corrected.