Fig. 4

Allelic variation and phylogenetic relationships of the VfTFL1a gene in Vicia faba varieties with different phenotypes. (a) Multiple sequence alignment of the coding sequence for VfTFL1a from 10 Vicia faba varieties, including four determinate phenotype varieties (in light green) and six indeterminate phenotype varieties, including the reference varieties Hedin and Tiffany (in red). The SNP (G/T) at the 26th bp, previously identified as a diagnostic marker distinguishing the two phenotypes is highlighted. In this dataset, the SNP differentiates only half of the determinate varieties from the indeterminate varieties. For alignment of the entire sequence (transcribed region of the gene as well as 500 bp up-and downstream of it) see Supplementary Figure S7. (b) Phylogenetic relationships among the 10 varieties based on the transcribed region of the gene and 500 bp upstream and downstream of the VfTFL1a gene. The tree shows mixed branching patterns and does not group varieties strictly by phenotype, with determinate (light green) and indeterminate phenotypes (white, including reference varieties in red). The tree was constructed using the Maximum Likelihood method with Neighbor-Joining and the Kimura 80 substitution model in CLC^42,43.