Fig. 1 | Scientific Reports

Fig. 1

From: Tcf21 modulates fibroblast activation and promotes cardiac fibrosis after injury via Pdgfrb signaling

Fig. 1

Tcf21 knockout (KO) fibroblasts show attenuated proliferation capacity and reduced Pdgfrb signaling. (a,b) 5-bromo-2′-deoxyuridine (BrdU) uptake decreased in Tcf21 KO fibroblasts (biologically n = 4). Scale bar, 200 µm. (c) Western blots of Smad2 and phosphorylated Smad2 (p-Smad2), with TGF-β (10 ng/mL) stimulation for 15 min (biologically n = 3). (d) Quantitative analysis of p-Smad2/Smad2. (e) Western blots of Erk and phosphorylated Erk (p-Erk), with PDGF-BB (20 ng/mL) stimulation for 15 min (biologically n = 3). (f) Quantitative analysis of p-Erk/Erk. (g) qRT-PCR, (h,i) Western blot analysis of Pdgfrb (biologically n = 3). (j) qRT-PCR analysis using human cardiac fibroblasts with TCF21 knockdown by CRISPR-CAS9 showing decreased expression of TCF21, POSTN, ACTA2, COL1A1, COL3A1, and PDGFRB (biologically n = 3).

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