Fig. 2
Tcf21 regulates extracellular matrix, proliferation, and inflammation-associated genes. (a,b) Results of gene expression cluster analysis obtained from DAVID 6.8 and Metascape showing altered expression of extracellular matrix and proliferation signaling genes in fibroblasts obtained from the heart of Tcf21 KO mice compared to those obtained from control mice (biologically n = 3). (c) Volcano plot shows that the expression of 440 genes was significantly altered by Tcf21. Upregulated genes in Tcf21 WT fibroblasts contain many extracellular matrix genes. Red dots represent significant genes upregulated in Tcf21 WT (P < 0.05). Blue dots represent significant genes downregulated in Tcf21 WT (P < 0.05). (d) Results of quantitative RT-PCR showing decreased expression of extracellular matrix and fibroblast activation genes in Tcf21 KO fibroblasts (biologically Control n = 15, iKO n = 24).
