Table 1 Details of the primers used for quantitative Real-Time PCR and common PCR assays in this study.
From: Effects of abiotic and biotic elicitors on tomato resistance to tomato brown rugose fruit virus
Target | Amplicon size (bp) | Annealing temperature (°C) | Nucleotide sequence | |
|---|---|---|---|---|
Forward (3’→5’) | Reverse (3’→5’) | |||
COI1 | 125 | 56.86 | 5’-AGTGAGAGGCTGCTGTTTC-3’ | 5’-CCATCGCTAAGAGATCACGAC-3’ |
PR1b | 125 | 58 | 5’-ACTACGCTACCAACCAATGTG-3’ | 5’- AGAAATGAACCACCATCCGT-3’ |
NPR1 | 131 | 56.3 | 5’-CCAAGTCTACAGAGGAAGGA-3’ | 5’-CAAATCATCGCCTGCCATAG-3’ |
LOC544055 | 112 | 56.8 | 5’-GTTGTTGAGACCTTTGCTGA-3’ | 5’-CAGTTGGGTCCTTCTTGTCA-3’ |
ToBRFV | 840 | 53 | 5’-GAAGTCCCGATGTCTGTAAGG-3’ | 5’-GTGCCTACGGATGTGTATGA-3’ |
