Fig. 1 | Scientific Reports

Fig. 1

From: Comparative analysis of 3D-culture techniques for multicellular colorectal tumour spheroids and development of a novel SW48 3D-model

Fig. 1

Representative images of CRC cell lines spheroids generated using different techniques and extracellular matrices. The cell lines tested were DLD1, HCT8, HCT116, LoVo, LS174T, SW48, SW480, and SW620. Cells were seeded at 105 cells/mL for liquid overlay on 1% (w/v) agarose, 3,000 cells in 30 µL drops for hanging drop, and 3,000 cells/well in U-bottom 96-well cell repellent plates. The concentrations of the different extracellular matrices were 2.5 mg/mL methylcellulose, 2.5% Matrigel, and 25 µg/mL collagen type I. All pictures were taken 72 h after cell seeding using a Leica DM6000B microscope with 4X objective.

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