Fig. 3 | Scientific Reports

Fig. 3

From: Comparative analysis of 3D-culture techniques for multicellular colorectal tumour spheroids and development of a novel SW48 3D-model

Fig. 3

Long-term cultured of CRC spheroids. (A) Representative images of CRC spheroids cultured over a week. All cell lines were cultured in U-bottom 96-well cell repellent plates with 2.5% Matrigel. Cells were seeded at 3,000 cells/well. All pictures were taken using a LEICA DM6000B microscope with 4X objective. (B) CRC spheroid area measurements using Image J from micrographs taken over one week. Every time point represents data from 8 individual spheroids. The grey shaded areas indicate the 95% confidence intervals of the Locally Estimated Scatterplot Smoothing (loess).

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