Fig. 5

SW48 compact spheroid models. (A) Representative images of MCTS generated in MT-plates treated with an anti-adherence rinsing solution. Cells were seeded at 3,000 cells per well in different conditions: no matrix, 25 µg/mL collagen type I, and 2.5% Matrigel. Pictures were taken using a LEICA DM6000B microscope with 4X objective, and analysed using an automated ImageJ script (Supplementary Fig. 4.2). The complete set of pictures are provided in the Supplementary File TimeSeries.zip. (B) Morphological analysis of SW48 spheroids cultured with no matrix, collagen I or Matrigel. The addition of collagen I or Matrigel generated smaller, rounder, smoother and more compact SW48 spheroids. P-values indicate the statistical significance of the effect of the matrix after correcting for time (multivariate linear regression, with time and matrix as explanatory variables). (C) Viability evaluation by DAPI staining of SW48 spheroids cultured for 72 h in anti-adherence treated MT-plates in absence of matrix, or with 2.5 mg/mL methylcellulose, 25 µg/mL collagen type I, or 2.5% Matrigel. MCTS were stained with DAPI (1 µg/mL) to label dead cells. Pictures were taken using a LEICA DM6000B microscope with 10X objective.