Fig. 2
Knockdown of NSUN2 expression results in decreased proliferation and increased apoptosis. (A) NSUN2 expression level in MM cell lines detected using RT-qPCR and Western blot. (B) NSUN2 expression level in mock and NSUN2-knockdown RPMI 8226 cells assessed using RT-qPCR and Western blot. (C) NSUN2 expression level in mock and NSUN2-knockdown MM.1 S cells assessed using RT-qPCR and Western blot. (D) Dot blots show the m5C signal relative to the methylene blue signal in mRNA from RPMI 8226 cells and MM.1 S cells after NSUN2 knockdown. (E) Cell proliferation determined by CCK-8 assays. (F) Colony formation assays show proliferation of Mock-, shNUSN2-RPMI 8226 cells and shNUSN2-MM.1 S cells. (G) Downregulation of NSUN2 increases the apoptotic rate in RPMI 8226 cells and MM.1 S cells. The statistical analysis of apoptosis percentage in Mock and shNSUN2 RPMI 8226 cells and MM.1 S cells induced in the absence (0%) and presence (10%) of FBS detected using FACS analysis. Data are represented as the mean ± SEM (n = 3 independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001. MM, multiple myeloma; NSUN2, NOP2/Sun RNA methyltransferase family member 2; RT-q-PCR, quantitative RT-PCR; CCK-8, Cell Counting Kit-8; SEM, standard error of the mean.
