Fig. 3
m5C modification pattern and RNA expression in mock and NSUN2-knockdown RPMI 8226 cells. (A) Integrative Genomics Viewer (IGV) showing the expression of NSUN2 in RPMI 8226 cells following NSUN2 knockdown. (B) Histogram showing the number of overexpressed and underexpressed differentially expressed genes (DEGs). (C) Volcano plot after NSUN2 knockdown. (D) Heatmap expression profile of 454 significantly dysregulated RNAs between mock and shNSUN2-RPMI8226 cells. (E) Statistical analysis of KEGG pathway enrichment for the DEGs. (F) Histogram showing the number of upregulated and downregulated m5C-methylated transcripts in mock and shNSUN2-RPMI8226 cells. (G) Statistical analysis of KEGG pathway enrichment for the differentially methylated genes (DMGs). NSUN2, NOP2/Sun RNA methyltransferase family member 2; KEGG, Kyoto Encyclopedia of Genes and Genomes.
