Table 7 Primers used for gene expression analysis.
From: Parental social environment has transgenerational effects on zebrafish (Danio rerio) growth
Gene | Gene Description | Primers |
|---|---|---|
il-1b78 | Interleukin-1 beta – pro-inflammatory cytokine | F: TGGACTTCGCAGCACAAAATG R: GTTCACTTCACGCTCTTGGATG |
mmp990 | Matrix metallopeptidase 9 – breakdown of extracellular matrix | F: CATTAAAGATGCCCTGATGTATCCC R: AGTGGTGGTCCGTGGTTGAG |
tnfa91 | Tumor necrosis factor alpha – pro-inflammatory cytokine | F: GCGCTTTTCTGAATCCTACG R: TGCCCAGTCTGTCTCCTTCT |
ghrelin64 | Ghrelin – orexigenic effect | F: GCATGTTTCTGCTCCTGTGTG R: AGATTCTGAAGCACGGGACC |
leptin64 | Leptin – anorexigenic effect | F: CGCTGACAAACCCATCCAAG R: CAGCTGGTCCAGATTATCGATCA |
cck64 | Cholecystokinin – release of digestive enzymes | F: CAGCTCTCTCTGCGTCTCTG R: TGCGGTATGACCCTTTGGTT |
ghra59 | Growth hormone receptor | F: TGCTGTGCGCTACAAAATGG R: GCTTCTGCAAAGGCTGATAGAAA |
ghrb59 | Growth hormone receptor | F: GAACTCAGAGTCCGGGCAAA R: AAAGACCAGCACAGCCGTAA |
igf1a59 | Insulin-like growth factor 1a | F: CAGGCAAATCTCCACGATCTC R: TTTGGTGTCCTGGGAATATCTGT |
ef1a92 | Elongation factor 1a – reference gene | F: TTGAGAAGAAAATCGGTGGTGCTG R: GAACGGTGTGATTGAGGGAAATTC |
