Fig. 2

Embryonic development following fertilization with unsorted or sex-sorted semen. COCs were allocated to two experimental groups and fertilized with unsorted or sex-sorted semen. (A) Presented is the proportion of oocytes that cleaved to 2-4-cell-stage 42–44 hpf. Presented is the proportion of blastocysts calculated from the total oocytes and from the cleaved embryos. Data are presented as means ± SEM. An asterisques above the bar indicates a significant difference between groups. *P < 0.05. (B) Presented is the distribution of normally vs. abnormally cleaved embryos in the unsorted and sex-sorted groups. (B′) Presented is the distribution of normally cleaved embryos according to the number of asynchronous cleavage events in unsorted and sex-sorted groups. Data analyzed using the chi-squared test were followed by the Pearson test. (C) Presented is the embryo kinetics. Data are presented in whisker plots and indicate the maximum and minimum values within the acceptable range defined by the two quartiles. Boxes indicate the 25th and 75th percentiles, and the middle-horizontal line indicates the median. The line inside the box is the median; the upper whisker denotes the 95th percentile values and the lower whisker denotes the 10th percentile. Data were analyzed using the Kruskal Wallis test, followed by Dunn’s posthoc test, which were used to compare the median value in the different stages of embryonic development. Different letters above the bar indicate significant differences between groups within each developmental stage. *P < 0.05.