Table 1 Primers used for PCR analysis.
From: Association between sex and the developmental morphokinetics of in vitro derived bovine embryos
Primer | Name | Primer | Sequence (5′→3′) | Size (bp) |
|---|---|---|---|---|
TSPY | Testis specific protein Y-linked 1 | Forward | GGTACAGGGCACGTCGAT | 153 |
Reverse | TGATGATCTCAGCAATCCTGTT | |||
BOV97M | Cattle Y-specific repeated DNA sequence | Forward | GATCACTATACATACACCACT | 143 |
Reverse | AAGGCTATGCTACACAAATTCTG | |||
AUTO | Autosomal | Forward | TGGAAGCAAAGAACCCCGCT | 217 |
Reverse | TCGTGAGAAACCGCACCCTG |
