Table 4 Validation of the kinetic sex prediction using the PCR-DNA template.
From: Association between sex and the developmental morphokinetics of in vitro derived bovine embryos
No. of kinetic combinations | Developmental stage | DNA validation | |||
|---|---|---|---|---|---|
Male | Female | ||||
% Truea (validate/predicted) | % Falseb (validated/predicted) | % Truea (validated/predicted) | % Falseb (validated/predicted) | ||
Single kinetic parameter | 6-cell stage | 63.3 (31/49) | 36.7 (18/49) | 63.4 (7/11) | 36.4 (4/11) |
7-cell stage | 59.3 (32/54) | 40.7 (22/54) | 50.0 (3/6) | 50.0 (3/6) | |
8-cell stage | 60.1 (31/51) | 39.2 (20/51) | 55.6 (5/9) | 44.4 (4/9) | |
Morula | 59.1 (26/44) | 40.9 (18/44) | 43.8 (7/16) | 56.2 (9/16) | |
Two kinetic parameters | 6- & 7-cell stages | 59.6 (28/47) | 40.4 (19/47) | 50.0 (2/4) | 50.0 (2/4) |
6- & 8-cell stages | 61.4 (27/44) | 38.6 (17/44) | 33.33 (1/3) | 67.7 (2/3) | |
6-cell stage & morula | 61.8 (21/34) | 38.2 (13/34) | 100.0 (2/2) | – | |
7- & 8-cell stages | 59.2 (29/49) | 40.8 (20/49) | 50.0 (2/4) | 50.0 (2/4) | |
7-cell stage & morula | 61.1 (22/36) | 38.9 (14/36) | – | – | |
8-cell stage & morula | 57.9 (22/38) | 42.1 (16/38) | 33.33 (1/3) | 67.7 (2/3) | |
Three kinetic parameters | 6-, 7-, & 8-cell stages | 61.4 (27/44) | 38.6 (17/44) | 33.33 (1/3) | 67.7 (2/3) |
6- & 7-cell stages & morula | 60.6 (20/33) | 39.4 (13/33) | – | – | |
6- and 8-cell stages & morula | 66.7 (22/33) | 33.3 (11/33) | 0 (0/1) | 100 (1/1) | |
7- and 8-cell & morula | 61.1 (22/36) | 38.9 (14/36) | – | – | |
Four kinetic parameters | 6-, 7-, & 8-cell stages & morula | 62.5 (20/32) | 37.5 (12/32) | – | – |
