Table 2 Comparison of results between micpcr/ngs and micpcr/nanopore sequencing using six culture-negative clinical samples.

From: Rapid and reliable species-level identification from clinical samples using 16S rRNA gene nanopore sequencing analysis

ID

Clinical picture

micPCR/NGS

micPCR/nanopore sequencing

Taxonomy

Quantification

Taxonomy

Quantification

1_T

Lymphadenopathy

Streptococcus spp.

< 25

Streptococcus dysgalactiae

< 25

2_T

Endocarditis

Cardiobacterium spp.

1,859

Cardiobacterium hominis

1,068

3_C

Endocarditis

Negative

0

Negative

0

4_A

Epidural abscess

Fusobacterium spp.

132,369

Fusobacterium necrophorum

67,054

5_C

Meningitis

Streptococcus spp.

< 25

Streptococcus pyogenes

< 25

6_P

Sepsis

Negative

0

Negative

0

  1. Quantification values represent the number of 16S rRNA gene copies/µl per DNA eluate.
  2. T tissue sample, C cerebrospinal fluid sample, A abscess sample, P EDTA plasma sample.
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