Fig. 11
From: Mechanodynamic brain on chip for studying human stem cell derived neuronal networks
Overview of hiPSC-derived neuron cultures in 2D (a) and 3D (f) BoC models on day 21. Hydrogels were removed from the reservoirs in the 3D BoC cultures. Co-localization of the post-synaptic marker HOMER-1 (c, h) and the pre-synaptic marker SYN1/2 (d, i) on axons. (e, j) All neuronal markers visualized together in panels (e) and (j). Neurons are labeled with SMI312 (axonal marker, orange), MAP2 (soma-dendritic marker, green), SYN1/2 (pre-synaptic marker, red), and HOMER-1 (post-synaptic marker, blue). Scale bars: 100 \(\upmu\)m. (k) Schematic illustration of synapse (created with Biorender). (l) Statistical comparison between 2D BoC and 3D BoC groups was performed using an unpaired two-tailed t-test (*p < 0.05, n=17) by GraphPad Prism.
