Fig. 2 | Scientific Reports

Fig. 2

From: Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway

Fig. 2

CDN treatment reduced the stemness of cancer stem cells in OS cells. (A)The formation and division patterns of spheroids were examined through electron microscopy. Scale bar: 5 μm. (B)Sphere formation assays revealed reduced self-renewal capacity in cells treated with CDN for a week. Scale bar: 200 μm. (C)Following the treatment of OS cells with CDN for 24 h, the levels of CSCs-labeled proteins CD133, SOX2, Nanog, and Oct4 were quantified using WB. (D)Following treatment, we detected the expression of marker proteins Oct3/4 and CD133 using immunofluorescence. Scale bar: 25 μm. *p < 0.05; **p < 0.01 and ***p < 0.001 vs. the untreated control.

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