Fig. 2

Suppression of adipogenesis in 3T3-L1 adipocytes by Bombycis Feces extract (BFE) treatment. (A) Schematic representation of experimental set-up. (B) Effects of BFE on adipogenesis and lipid accumulation assessed by Oil Red O staining and quantification using Image J software (40x: left, 100 × right, scale bar: 100 μm). (C) qPCR analysis of adipogenesis-associated gene expression in 3T3-L1 adipocytes differentiated for 9 days in the presence and absence of BFE. All data are representative of 2–3 independent experiments. Data are presented as the mean ± SD. *p < 0.05; *** p < 0.001 indicate significant differences between the indicated groups.