Fig. 6
GnRH immunofluorescence in the hypothalamus of male rats exposed to sodium arsenite. Fluorescent micrographs showing gonadotropin-releasing hormone (GnRH) immunoreactivity in the hypothalamic region of rats treated with sodium arsenite at concentrations of 0 (a), 1 (b), 5 (c), 25 (d), and 50 mg/L (e) via drinking water. Alexa Fluor 488-conjugated mouse anti-GnRH secondary antibodies (Thermo Fisher Scientific, USA) were used for detection. Images were captured using an Optika fluorescence microscope at 20× magnification, with excitation at ~ 488 nm and emission at ~ 510–540 nm. Exposure time and gain were kept constant across all samples. Arrows indicate reduced GnRH expression with increasing arsenic concentration.
