Fig. 5

DIO enhances intestinal bacterial translocation to the pancreas as assessed by FISH and deep learning-assisted quantification. (a) Detection of indigenous bacteria within the jejunum and pancreas was achieved using eubacterial FISH probe labeling (Scale bar: 50 μm); (b, c) Quantitative analysis of indigenous bacterial abundance in the jejunum and pancreas, with visualization by FISH and enumeration facilitated by deep learning-assisted image analysis. Data points represent mean ± standard deviation from independent experiments, with n = 6 mice per group. Statistical significance is denoted as ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 by two-way Anova with Tukey’s multiple comparisons test.