Fig. 6

Accumulation of lipids in the intestine significantly compromised mucosal permeability in DIO mice. (a, b) Lipid droplets in the intestinal villi stained with oil red O (ORO) (scale bar: 50 μm) and its quantification; (c, d) Lipid deposition ORO staining (scale bar: 1 mm) and quantification on the small intestinal mucosal surfaces; (e–g) Real-time quantitative reverse transcription PCR (RT-qPCR) analysis of jejunum mRNA expression of the claudin 3, claudin 5, and mucin 2 genes; (h) Secretion of claudin 3 in the jejunum by immunofluorescence staining (Scale bar: 10 μm). Blue: DAPI, Green: claudin 3. Data points represent mean ± standard deviation from independent experiments, with n = 6 mice per group. Statistical significance is denoted as ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 by two-way Anova with Tukey’s multiple comparisons test.