Fig. 3

mRNA expression of ECS and dopaminergic-related genes in HIP and PFC of PTE offspring at PND 7. (A) CB2R mRNA expression in the HIP was significantly higher in Sub Veh compared to Dom Veh, but downregulated in both Dom-PTE and Sub-PTE offspring relative to their respective controls. (B) FAAH expression in the HIP was elevated in Sub Veh compared to Dom Veh and significantly reduced in Sub-PTE compared to Sub Veh. (C) D5R mRNA expression in HIP was downregulated in Dom-PTE and Sub-PTE offspring compared to Dom Veh and Sub Veh offspring, respectively. (D) In the PFC, CB1R expression was downregulated in Sub-PTE compared to Sub Veh. (E) CB2R expression in the PFC was significantly upregulated in Sub-PTE compared to Sub Veh. (F) FAAH expression in the PFC was downregulated in Sub-PTE compared to Sub Veh. D1R (G) and D2R (H) in PFC are higher in the Dom strain (Veh) compared to Sub and downregulated in Dom-PTE compared to Dom Veh offspring, respectively. Data are presented as mean ± SEM (n = 4–5/group). Statistical significance was determined using two-way ANOVA with Sidak’s correction and is denoted as * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001. Significant differences identified via student’s t-test are denoted by p-value as # at p < 0.05.