Fig. 3 | Scientific Reports

Fig. 3

From: Harnessing electroacupuncture: a promising strategy against sleep deprivation-exacerbated post-cardiac arrest brain injury

Fig. 3

Electroacupuncture restores SD-compromised EAAT2/calcium signaling axis in post-cardiac arrest neurodegeneration. (A-B) Representative immunofluorescent images of EAAT2 (green) and astroglial marker GFAP (red) in hippocampal CA1 and prefrontal cortex regions, Nuclei counterstained with DAPI (blue). (C-H) Quantitative analysis of EAAT2 and GFAP fluorescence intensity, and GFAP-positive area fraction in hippocampus and prefrontal cortex. (I, M) Western blot membranes showing calcium signaling proteins (CAMKIV, p-CREB, BDNF) with loading controls (GAPDH/β-actin) in hippocampus and prefrontal cortex lysates. (J-L, N-P) Densitometric quantification of CAMKIV, p-CREB, and BDNF expression levels in hippocampus and prefrontal cortex.

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