Fig. 2 | Scientific Reports

Fig. 2

From: Irradiation of muscle precursor cells impairs the proliferative and angiogenic functions of their extracellular vesicles

Fig. 2

Extracellular vesicle characterization. Conditioned media was collected from non-irradiated MPCs or irradiated MPCs after 48-hour incubation in serum-free media. EVs from non-irradiated MPCs (NoRad-EVs) or EVs from irradiated MPCs (Rad-EVs) were isolated from the conditioned media using centrifugation and evaluated using (a) Transmission Electron Microscopy to assess size and morphology (Scale bar = 100 nm), (b) Nanoparticle Tracking Analysis to assess particle size distribution, (c) Vesicle mean size comparison, and (d) Protein concentration quantification using bicinchoninic acid protein assay. Results are reported as mean ± standard deviation. (n = 3)

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