Fig. 5 | Scientific Reports

Fig. 5

From: N,N-Diethylacetamide and N,N-Dipropylacetamide inhibit the NF-kB pathway in in vitro, ex vivo and in vivo models of inflammation-induced preterm birth

Fig. 5

DEA suppresses LPS-induced cytokine and chemokine secretion from human placental explants. Human placental explants were processed as described previously and pre-treated with DEA or BAY 11-7082 (25 µM) for 2 h. Then LPS was added at a final concentration of 1 µg/ml and explants were incubated for 20 h. After 20 h treatment, supernatants were collected and evaluated for various cytokines and chemokines. The levels of (A) TNF-α, (B) IL-6, (C) GM-CSF, (D) IL-8, (E) MCP-1 and (F) IL-10 after the treatment with DEA were determined using sandwich ELISA. Data shown are mean ± SEM of four independent experiments performed in duplicate. Likewise, ELISA was performed in duplicate. To test for significant effects of each concentration, ANOVA was performed followed by Tukey’s multiple comparison post hoc analysis using GraphPad Prism 6 software. *P < 0.05, ***P < 0.001 and ****P < 0.0001.

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