Fig. 1
From: Metformin lotion as a novel approach to prevent tendinopathy induced by mechanical overuse
Met lotion inhibits ITR-induced HMGB1 release into the tendon matrix in tissue sections analyzed without Triton X-100 treatment. Without Triton X-100 treatment, only HMGB1 released to tendon matrix is positively stained. The results indicate that ITR induces HMGB1 release into the tendon matrix (red fluorescence: b–d), which is inhibited by ML (e–l). Semi-quantitative analysis confirms these results, showing that ML applications significantly decrease positively stained cells in the matrix, with 3% ML showing a 5.9-fold decrease and 6% ML showing minimal positive staining (m). c,g,k: Merged images of(a,b,e,f; and i,j respectively. d,h,l are enlarged images of c,g,k respectively. m: Semi-quantitative results from 45 images (15 sections from 5 mice per group) are presented as mean ± SD (n = 5). ML: Met Lotion. Scale bars: 50 μm (yellow); 15 μm (white).
