Fig. 2
From: Metformin lotion as a novel approach to prevent tendinopathy induced by mechanical overuse
Met lotion inhibits ITR-induced HMGB1 release into the tendon matrix in tissue sections analyzed with Triton X-100 treatment. In tendon tissues treated with Triton X-100, which allows staining to detect HMGB1 both in the tendon matrix and within cell nuclei, staining results show that high levels of HMGB1 remain localized in the cell nuclei of ML-treated tendons (e–l), whereas ITR releases most of HMGB1 from nuclei (b–d). Semi-quantitative analysis of immunostaining results shows a 4-fold increase in positively stained nuclei in ML-applied samples compared to ITR-only samples (m). c,g,k: Merged images of a,b;e,f; and i,j respectively. d,h,l are enlarged images of c,g,k, respectively. m: Semi-quantitative results from 45 images (15 sections from 5 mice per group) are presented as mean ± SD (n = 5). ML: Met Lotion. Scale bars: 50 μm (yellow); 15 μm (white).
