Fig. 4
miR-9 could antagonize the local inflammatory response. The expressions of IL-1β, IL-6, TNF-α, MPO, p-P65, P50, and CD68 were significantly decreased by miR-9a-5p agomir. Inversely, the expressions of SOD1, SOD2, IκBα, and IκBβ were significantly increased by miR-9a-5p agomir, compared with SAP + PBS or miR-9a-5p control group, by gPCR (A,B), Western-blot (C,D) and IHC (E,F). Data are shown as mean ± SD for at least 3 separate experiments. %P < 0.05, compared with NC, #p < 0.05 and ###p < 0.001, compared with NC, *p < 0.05, **p < 0.01 and ***p < 0.001, compared with SAP + PBS, &p < 0.05, &&p < 0.01, and &&&p < 0.001, compared with miR-9a-5p control by using paired t test. SOD, Superoxide Dismutase, gPCR, General PCR, IHC, immunohistochemistry.
