Table 2 Evaluation of iELISA results of the Recombinant proteins.

From: Application of Brucella type IV secretion proteins VirB1, VirB5, and VirB6 in the serological diagnosis of brucellosis

Antigen

AUC

Cut-off

Sensitivity

Specificity

Positive

Negative

Accuracy

PPV

NPV

value

(95%CI)

(95%CI)

TP

FN

TN

FP

(%)

(%)

(%)

rVirB1

0.9948

> 0.3149

0.9900

0.9479

99

5

91

1

96.94

99

94.79

(0.9887 to 1.000)

(0.9455 to 0.9995)

(0.8838 to 0.9776)

rVirB5

0.9855

> 0.3121

0.9500

0.9375

95

6

90

5

94.39

95

93.75

(0.9736 to 0.9974)

(0.8882 to 0.9785)

(0.8703 to 0.9710)

rVirB6

0.9808

> 0.2345

0.9500

0.9271

95

7

89

5

93.85

95

92.71

(0.9665 to 0.9952)

(0.8882 to 0.9785)

(0.8571 to 0.9642)

LPS

0.9999

> 0.1953

0.9900

1.000

99

1

96

0

99.49

100

98.97

(0.9995 to 1.000)

(0.9455 to 0.9995)

(0.9615 to 1.000)

  1. TP, true positives; TN, true negatives; FP, false positives; FN, false negatives; Accuracy, (TP + TN/TP + FN + TN + FP) ×100; PPV, positive predictive value (TP/TP + FP) ×100; NPV, negative predictive value (TN/TN + FN) ×100.