Table 2 Biophysical properties of Nav1.4/WT and E452K mutant at 15 ˚C, 10 ˚C.
hNav1.4/WT | hNav1.4/E452K | P Value t-test two-tailed | |
|---|---|---|---|
Peak current 15˚C (pA/pF) | −278.4 ± 41.0 (n = 8) | −128.8 ± 22.7** (n = 7) | 0.0059 |
Activation | |||
V0.5 (mV) | −12.2 ± 1.7 (n = 8) | −12.5 ± 1.1 (n = 7) | 0.69 |
k (mV) | 7.7 ± 0.3 (n = 8) | 8.2 ± 0.3 (n = 7) | 0.40 |
Inactivation | |||
V0.5 (mV) | −66.8 ± 2.0 (n = 6) | −65.0 ± 1.1 (n = 7) | 0.37 |
k (mV) | −6.5 ± 0.4 (n = 6) | −5.9 ± 0.1 (n = 7) | 0.29 |
Recovery | |||
τ (ms) | 1.3 ± 0.1 (n = 8) | 1.2 ± 0.0 (n = 8) | 0.28 |
Window Current | |||
Total area | 2.6 | 3.5 | |
Peak | 0.054 | 0.088 | |
Peak current 10˚C (pA/pF) | −134.7 ± 19.8 (n = 10) | −69.5 ± 9.4** (n = 7) | 0.0097 |
Activation | |||
V0.5 (mV) | −8.1 ± 1.2 (n = 10) | −7.2 ± 1.3 (n = 7) | 0.54 |
k (mV) | 8.0 ± 0.3 (n = 10) | 8.2 ± 0.4 (n = 7) | 0.89 |
Inactivation | |||
V0.5 (mV) | −69.8 ± 1.0 (n = 4) | −66.0 ± 0.6* (n = 7) | 0.012 |
k (mV) | −6.1 ± 0.1 (n = 4) | −6.0 ± 0.3 (n = 7) | 0.89 |
Recovery | |||
τ (ms) | 3.20 ± 0.46 (n = 6) | 2.49 ± 0.29 (n = 4) | 0.48 |
Window Current | |||
Total area | 1.14 | 1.94 | |
Peak | 0.026 | 0.047 |
