Fig. 2
From: AMIGO2 accelerates tumor progression by inducing a cancer stem cell-like phenotype
AMIGO2 expression is required for self-renewing sphere formation from single cancer cells in three-dimensional cultures MKN45 (a and b) and DLD-1 (c and d) cells and their transfectants were suspended in a medium containing 1.4% methylcellulose and plated in a 96-well plate (1 × 103 cells/well). The number of spheres greater than 50 μm in diameter was counted (a and c). The mean ± s.d. of n = 6 independent experiments is shown. The spheres were photographed (b and d) for day 12 for MKN45 cells and day 10 for DLD-1 cells after seeding. Representative images were presented from three experiments with similar results. Scale bars = 200 μm.
