Fig. 4
YM–254,890 attenuates the urothelial cell-mediated local bladder contractions. (a) Representative recordings of the contractions evoked by 70 mM KCl and blue light (20 mW, 5 s). Blue light evoked urothelial cell-mediated local bladder contractions were recorded in the presence of vehicle and YM–254,890 (100 nM). (b) Summarized data showing the inhibitory effects of YM–254,890 (100 nM) on the urothelial cell-mediated local bladder contractions. (c) Summarized data of the percentage of inhibition of the urothelial cell-mediated local bladder contractions by YM–254,890 (100 nM). (d) Representative recordings of the contractions evoked by 70 mM KCl and carbachol (3 µM) and ATP (50 µM). (e, f) Summarized data showing the inhibitory effects of YM–254,890 (100 nM) on the carbachol (3 µM) and ATP (50 µM)-evoked contractile responses. The amplitude of the urothelial cell-mediated local bladder contractions was expressed as a percentage of the 70 mM KCl-evoked contractions in the same preparation. Each point represents the mean ± SD of the mean (n = 6 for YM–254890 on the urothelial cell mediated contractions and n = 5 for YM–254890 on carbachol and ATP-evoked contractions). *Significantly different (p = 0.045) from the corresponding control values. Blue bars correspond to light stimulation. NS = non-significant (p = 0.860). Blue bars correspond to light stimulation
