Table 3 Performance evaluations of the embryo test and our qPCR method in the detection of Ustilago nuda infections across six commercially available seed lots.

From: A new molecular seed assay to predict Ustilago nuda field infection levels

 

Sensitivity

Specificity

Precision

False discovery rate

Accuracy

Embryo test

0.0

1.0

–

–

0.6

qPCR method

1.0

1.0

1.0

0.0

1.0

  1. The performance metrics include sensitivity, specificity, precision, false discovery rate, and accuracy. These performance metrics were based on the six seed lots’ agreement between their predicted values, which classified the seed lot as above or below the method-specific tolerance threshold, and their actual values based on the classification of the infections observed in the field as above or below its tolerance threshold (Table 2). The outcome of each seed lot’s classification according to the confusion matrices (Supplementary Information, Table 8) was used to evaluate the laboratory methods’ performance. Metrics marked with “–” were incalculable due to the absence of positive test results in the embryo test.
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