Fig. 3
The effect of UBE2S on the expression level of TRAF6 and the activity of MAPK and PI3K/AKT pathways in vivo. (A) Animal experiments demonstrate larger volumes of the transplanted tumor with UBE2S knockdown (n = 5); (B) Overexpression of UBE2S reduces the volumes of the transplanted tumor (n = 5); (C) Volume measurement in the growth of transplanted tumors in nude mice(n = 5); (D) RNA analysis of transplanted tumor tissue reveals that both UBE2S knockdown and overexpression have no effects on the transcription level of TRAF6 (n = 5); (E) Protein analysis of transplanted tumor tissues reveals an impact of UBE2S on TRAF6 protein (n = 5); (F) Immunofluorescence of transplanted tumors for the UBE2S and TRAF6 protein expression ((n = 5, 400×); (G) Statistical analysis of immunohistochemistry of transplanted tumors for UBE2S and TRAF6 proteins with UBE2S knockdown and overexpression; (H) In immunohistochemistry of nude mouse transplanted tumors, UBE2S knockdown results in an increase in the expression of TRAF6, p-AKT, and p-ERK proteins (n = 5, 400×); (I) In immunohistochemistry of nude mouse transplanted tumors, UBE2S overexpression results in a decrease in the expression of TRAF6, p-AKT, and p-ERK proteins(n = 5, 400×). *P < 0.05, **P < 0.01, ***P < 0.001, ‘ns’ represents no statistical significance.
