Fig. 2
From: Investigating the effects of miR-526b and miR-655 on doxorubicin sensitivity in breast cancer
Analysis of cell viability after DOX exposure. (A) Dose-response curve for DOX-treated MCF7. (B) Timeline of treatment and recovery (created with BioRender.com). (C) Viability of MCF7, MCF7-miR526b, MCF7-miR655 and MCF7-COX2 cells after treatment. Data represented as mean +/- SD, (n = 3, ****p < 0.0001, one-way ANOVA, Dunnett’s post-hoc). (D) p53, (E) p21 and (F) PUMA gene expression. Data shown as mean +/- SD (n = 4 for MCF7 and n = 3 for MCF7-miR526b, MCF7-miR655 and MCF7-COX2. DOX: MCF7 vs. MCF7-miR655 *p = 0.0229 in (D), in DMSO: MCF7 vs. MCF7-miR526b ***p = 0.0006, MCF7 vs. MCF7-COX2 ****p < 0.0001; in DOX: MCF7 vs. MCF7-COX2 *p = 0.0328; in DOX + Rest: MCF7 vs. MCF7-COX2 *p = 0.0490 in (E), in DMSO: MCF7 vs. MCF7-COX2 *p = 0.0251; in DOX: MCF7 vs. MCF7-miR526b *p = 0.0443, MCF7 vs. MCF7-miR655 ***p = 0.0012; in DOX + Rest: MCF7 vs. MCF7-miR655 *p = 0.0323, MCF7 vs. MCF7-COX2 **p = 0.0012 in (F), one-way ANOVA, Dunnett’s post-hoc). (G) Western blot images and quantification for (H) p53, (I) p21, (J) p-AKT(Ser473) and (K) PARP cleavage. For full blot images, see Supplementary Figs. S2-S6. Data presented as mean +/- SD (For p53, n = 4 for MCF7, MCF7-miR526b, n = 3 for MCF7-miR655 and MCF7-COX2. MCF7: DMSO vs. DOX **p = 0.0044, MCF7-miR526b: in DMSO vs. DOX **p = 0.0025, MCF7-miR655: DMSO vs. DOX *p = 0.0142, DMSO vs. DOX + Rest *p = 0.0367, MCF7-COX2: DMSO vs. DOX ***p = 0.0003, DOX vs. DOX + Rest ****p < 0.0001 in (H). For p21, n = 5 for MCF7, MCF7-miR526b, n = 4 for MCF7-miR655 and n = 3 for MCF7-COX2. MCF7: DMSO vs. DOX *p = 0.0369, MCF7-miR526b: DMSO vs. DOX **p = 0.0024, DOX vs. DOX + Rest *p = 0.0316, MCF7-miR655: DMSO vs. DOX **p = 0.0023, DOX vs. DOX + Rest *p = 0.0167, MCF7-COX2: DMSO vs. DOX **p = 0.0035, DOX vs. DOX + Rest *p = 0.0273 in (I), one-way ANOVA with Tukey’s post-hoc. For p-AKT/AKT, n = 3 for all cell lines. DOX: MCF7 vs. MCF7-COX2 *p = 0.0420 and MCF7-COX2: DMSO vs. DOX *p = 0.0182 in (J). For the fraction of cleaved PARP measurement, n = 3 for all cell lines. MCF7-miR526b: DMSO vs. DOX + Rest *p = 0.0318 and DOX vs. DOX + Rest *p = 0.0491 in (K), two-way ANOVA with Tukey’s post-hoc). Non-significant comparisons (p > 0.05) not labelled.
