Fig. 1

Volumetric analyses reveal that Setd2 cKO brains are smaller. Volumetric model-based segmentation analyses of Setd2 Ctrl (A, D, G) and cKO (B, E, H) brains were performed using the AMBMC whole brain atlas, and representative images of Ctrl and cKO brains were overlaid to show differences in volume (C, F, I). Volume of each structure, differences in volume, and normalised structural volume are shown in J. The corpus callosum, hippocampus, neocortex and thalamus, among other sub-substructures, were significantly reduced in absolute volume, as was the entire brain. Further delineation of structures and their associated volumes are shown in Supp. File 1. All data in J were analysed using multiple unpaired t-tests with a FDR of 5%. All data are represented as mean ± SEM. All values are rounded to 2 decimal places. Differences in (normalised) volume are represented as Ctrl – cKO. Scale bar represents 1 mm. CC = corpus callosum. For all analyses, n = 10 and n = 9 were used for Ctrl and cKO brains respectively.