Fig. 3

Setd2 cKO mice exhibit abnormal bilateral hippocampal connectivity. Representative tractography of Setd2 Ctrl (A–C) and cKO (D–F) brains in coronal (A, D), sagittal (B, E) and transverse (C, F) planes. All tracts presented represent connectivity between the left hippocampal body (HpL) and the right hippocampal body (HpR), with only tracts passing through the fornix (For) being shown. Diffusion tensor imaging (DTI) metrics (axial diffusivity (AD), apparent diffusion coefficient (ADC), fractional anisotropy (FA) and radial diffusivity (RD)) are represented (G). All metrics but AD were significantly altered, with significant increases being seen in ADC and RD and significant decreases in FA. Colours for DTI represent the following directionality: green is left–right oriented, red is dorso-ventral oriented, blue is rostro-caudal oriented; oblique fibre orientations are a combination of these colours. * p < 0.05. For all analyses, n = 10 and n = 9 were used for Ctrl and cKO brains respectively.