Fig. 8

Setd2 cKO brains show alterations in hippocampal neuronal populations. Coronal sections taken at the level of the dentate gyrus of the hippocampus from Setd2 control (A–B’”) and cKO (C–D’”) brains, revealing the expression of PROX1 (red), Dcx (green) and DAPI (white). Quantification of (E) the PROX1⁺ area of each blade of the dentate gyrus, (F) the density of PROX1 cells within the DG, (G) the number of Dcx⁺ neuroblasts and (H) the length of each blade of the cKO DG occupied by Dcx⁺ neuroblasts as a percentage of control brains. ns = not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. Scale bar in D’” represents 100 µm for all large panels, and 20 µm for all insets. For all analyses, an n = 5 was used for each genotype.