Fig. 2
Differentiation of BMSCs into CNS cells in breastfed wild-type mice. (a) Immunofluorescence images showing BMSC origin YFP+, CFP + and DsRed + cells in cleared brain sections that were stained against anti-GFP, anti-MBP and anti-GFAP for neurons, oligodendrocytes, and astrocytes respectively. (b) Flow cytometry analysis of brain tissues. Left panel shows the YFP + cells (neurons) and the right panel shows the CFP+ (oligodendrocytes) and DsRed+ (astrocytes) cells. (c) Quantitative analysis of flow cytometry of 2-months old Prism-BF compared to WT-BF (n = 5). (d) Quantitative analysis of confocal microscopy images of oligodendrocytes, neurons, and astrocytes obtained from three randomized ROIs (each 8 × 106µm2) per animal in the striatum of 2-months old Prism-BF compared to WT-BF (n = 5). The mean value of the three ROIs was calculated for each animal and used as a single biological replicate in the analysis, error bars represents standard deviation.
