Fig. 3
Differentiation of BMSC into CNS cells in MCAO induced breastfed wild-type mice. (a) Representative image showing TO-PRO3 staining for cleared brain section and autofluorescence indicating infarct zone after MCAO/R. (b,c) Immunofluorescence images show YFP+, CFP+, and DsRed + cells in cleared brain sections after MCAO/R in both the ipsilateral and contralateral hemispheres, stained using anti-GFP, anti-MBP and anti-GFAP antibodies to identify neurons, oligodendrocytes, and astrocytes respectively. (d) Flow cytometry analysis of ipsilateral and contralateral hemispheres. Top panels show the YFP + cells (neurons) and the bottom panels show the CFP+ (oligodendrocytes) and DsRed+ (astrocytes) cells. (e) Quantitative analysis of flow cytometry of contralateral and ipsilateral hemispheres in Prism-BF I/R group (n = 5). (f) Quantitative analysis was performed on confocal microscopy images of oligodendrocytes, neurons, and astrocytes obtained from three randomized ROIs (each 8 × 10⁶ µm³) per animal in the striatum of both contralateral and ipsilateral hemispheres in the Prism-BF I/R group (n = 5). The mean value of the three ROIs was calculated for each animal and used as a single biological replicate in the analysis, error bars represent standard deviation.
