Fig. 3

Prob attenuates inflammation and oxidative stress in LPS-induced BV2 cells. A BV2 cells were treated with various concentrations of Prob (0, 50, 100, 200, 400, 800, 1600, and 3200 µM) for 24 h, and cell viability was evaluated with CCK-8. B SOD levels were measured using ELISA and MDA levels were measured using microwell plate method. C Levels of TNF-α and IL-1β in the cell culture supernatant were measured using ELISA, and NO levels were assessed with microwell plate method. D ROS levels were determined using FCM. Data are presented as mean ± SD (n = 3). ^#P < 0.05, ^##P < 0.01 vs. LPS group; *P < 0.05, **P < 0.01 vs. control group.